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Test Code 6010 Factor X Assay

Specimen Containers and Fixatives

3.2% Sodium Citrate Blue Top Tube (2nd Tube Method)

Specimen Required

NorthShore Lab Services: draw 5.0 mL blood as decribed in CLIENT SPECIAL INSTRUCTIONS, centrifuge and submit two plastic vials with 1.0 mL of frozen plasma in each.
Hospital Setting: submit 5.0 mL of blood drawn as described in CLIENT SPECIAL INSTRUCTIONS.

Client Special Instructions

The specimen must be double centrifuged for special coagulation testing (e.g. lupus, protein C,

S etc.). Centrifuge specimens immediately (must be spun within 2 hours of collection). Carefully

remove plasma from cells avoiding platelets or red cells. Dispense into a plastic tube and centrifuge

plasma for 10 minutes. Remove the top portion of the plasma leaving approximately 250μl in the

bottom to discard. The double – centrifuged plasma should be aliquoted (1ml each) into clearly

labeled plastic tubes. Plastic tubes should be labeled with the patient's name, date of drawing, and

specimen number. The specimen should never come in contact with glass. If plasma cannot be sent to Evanston Special Coag lab within 4 hours of collection, freeze specimen and send it on dry ice.

Day(s) and Time(s) Performed


Monday – Friday (7AM – 3PM), 48 hours

Reference Values

59-151% activity

Method Name

Clotting Assay

CPT Code Information