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Test Code 1721 NT-ProBNP

Specimen Containers and Fixatives

Light Green Top Tube with lithium heparin (PST)


For add-on testing, refer to link below for acceptable tube types:

Acceptable Tube Types for Chemistry Tests

Specimen Required

NorthShore Lab Services: draw 0.8 mL (0.5 min) blood, centrifuge and submit 0.3 mL (0.2 min) refrigerated plasma.

Hospital setting: submit 0.8 mL (0.5 min) blood.


For sample stability, refer to the link below:

Sample Stability Chart for Chemistry Analytes

Day(s) and Time(s) Performed


Daily, 24 hours

Reference Values

<300 pg/mL


NT-ProBNP values consistent with acute congestive heart (CHF) are the following:

<50 years: >450 pg/mL

50 – 75 years: >900 pg/mL

>75 yeard: >1800 pg/mL


NT-ProBNP levels are used to differentiate between congestive heart failure (CHF) and other conditions, such as pulmonary diseases, that produce similar symptoms and to monitor treatment for CHF. In general NT-ProBNP levels correlate with the New York Heart Association System for the classification of CHF in that higher NT-ProBNP levels predict a higher class on the New York Heart Association System. The NT-ProBNP molecule has a short half-life so NT-ProBNP levels decrease within hours of successful therapy.

Method Name

Electrochemiluminescense Immunoassay (ECLIA), sandwich

CPT Code Information


LOINC Code Information



Patient should avoid biotin containing supplements for 8 – 12 hours before the blood sample is collected.